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![]() This study traced the molecular characteristics of the most recent isolates in China and aimed to provide a basis for the prevention and treatment of PEDV. However, vaccines do not provide sufficient protection against currently circulating strains due to viral mutations. epidemic diarrhea virus (PEDV) variant strains cause great economic losses to the global swine industry. 6 Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China. 5 Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, Northeast Agricultural University, Harbin, 150030, People's Republic of China. 4 College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China. 3 Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001, People's Republic of China.Ltd., Beijng, 100070, People's Republic of China. ![]() 1 College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.What is the isoelectric point (pl) of PPO? Based on the pl, will PPO have a neutral, negative or positive charge at a physiological pH of 7. Paste the amino acid sequence into the box and press the Click here to compute pl/Mw button e. Open a new window and set the browser to /compute_pi. This will produce a version of the amino acid sequence that does not contain numbers, Highlight and copy and this sequence, without including the identifier information at the beginning.ĭ. At the top of this sequence, select the FASTA string button. You should now be at a page that shows your single letter amino acid sequence for PPO. Under the Sequences section in the information block, select the PDB- Europe hyperlink. On the 2phm tab, select the Sequences hyperlink. The isoelectric point, or pl, is the pH at which a protein has a zero net charge. How many disulfide bonds does the PPO structure possess. cell cytosol), or in a hydrophobic one (e.g. Is the surface of PPO largely hydrophilic or hydrophobic? Based on the surface properties, do you think it would be more soluble in an aqueous environment (c.g. Allowing the molecule to spin may also be useful (1 pt) 4. How would you describe the core of PPO? Check out the hydrophobic/polar view with the slab option. Large hydrophobic patches on the surface may be sites of protein- protein interaction. In soluble proteins, hydrophobic residues are frequently found buried in the cores of proteins. Does polyphenoloxidase contain any ligands? If so, what are they? (1 pt) 3. Fill in the following table for PPO (4 pt) No. There are three components to the webpage:(1) the molecule visualization window on the right-hand side of the page (2) A command block which allows you to manipulate views of your structure at the top left of the page, and (3) an information block at the bottom left of the page which allows you to find outġ. ![]() After several seconds, the structure of polyphenoloxidase should load. Enter polyphenoloxidase's Protein Database file ID, 2PHM, into the Enter a PDB identification code here box and return/enter. You should arrive at the FirstGlance in Jmol page. Your browser must have the Java software installed to work (most browsers already have this software installed). ![]() To begin, start your internet browser and go to. ![]() On 23 December 2010, Gol Airlines started an operational partnership with Brazilian carrier Passaredo Linhas Aéreas. Gol later started also using the base to service aircraft from other airlines, and as of 2013 it also serviced airplanes from Azul Brazilian Airlines and Copa Airlines. Originally opened in 2006 with the capacity to service 60 aircraft per year (mainly Boeing 737 and 767), the expansion allowed Gol to increase the number to 120. On 18 March 2010 Gol unveiled the expansion of its maintenance base located at Belo Horizonte/Confins - Tancredo Neves International Airport. Following this trend, on 28 September 2011 Gol and Aerolíneas Argentinas announced the intention to implement a codeshare, feeder and frequent flyer programs agreement on a date yet to be announced and pending approval from the governments of Brazil and Argentina. However, on 6 October 2010, the airline announced a change in position by denying any interest in joining an alliance, preferring to remain independent and to establish a "patchwork of code-sharing agreements.". On 24 February 2010 Gol announced it was in "advanced talks" to join the Oneworld alliance, which would allow it to catch up with rival TAM, a recent member of Star Alliance. Instead, TAP Portugal chose to cooperate with TAM Airlines. In 2007, Gol was supposed to begin a code-share agreement with TAP Portugal, opening the European market to the Brazilian airline, and the internal Brazilian market to the Portuguese airline (the largest foreign airline in Brazil). The growth in GOL's stock price made the Constantino family a member of the Forbes magazine billionaire list in 2005. In 2007, Gol was owned by AeroPar Participações (75.5%), Venture (17.6%), American International Group (5.4%) and Air France-KLM (1.5%). Constantino Júnior was responsible for building the business next to the vice president of the company, David Barioni. Grupo Áurea in turn is owned by the Constantino family. It is a subsidiary of the Brazilian conglomerate Grupo Áurea, based in Minas Gerais state, which has other transportation interests, including Brazil's largest long-distance bus company. and started operations on 15 January 2001 with a flight from Brasília to São Paulo. The airline was established in 2000 as Gol Transportes Aéreos S.A. Gol ticket counter at Brasilia International Airport (in Brazilian Portuguese) New Gol, New times on air. The company's name is a Brazilian Portuguese word borrowing from the English word "goal" from Association football. The company is traded on the New York Stock Exchange as "GOL Linhas Aéreas Inteligentes S.A.". Gol refers to itself as GOL Intelligent Airlines ( GOL Linhas Aéreas Inteligentes in Portuguese) as a slogan. Gol also has focus operations at Rio de Janeiro-Santos Dumont Airport, São Paulo-Congonhas Airport, Salgado Filho International Airport in Porto Alegre. ![]() Its main hubs are São Paulo–Guarulhos International Airport, Rio de Janeiro–Galeão International Airport and Tancredo Neves International Airport near Belo Horizonte. Gol operates a growing domestic and international scheduled network. It also owns the brand Varig, although now that name refers to what is informally known as the "new" Varig, founded in 2006, not to the extinct "old" Varig airline, founded in 1927. Gol competes in Brazil and other South American countries primarily with LATAM Brasil, and Azul. According to the National Civil Aviation Agency of Brazil (ANAC), between January and December 2019 Gol had 37.7% of the domestic and 3.8% of the international market shares in terms of passengers per kilometer flown, making it the largest domestic and third largest international airline in Brazil. Gol Linhas Aéreas Inteligentes S.A ( "Gol Intelligent Airlines S.A." also known as VRG Linhas Aéreas S/A) is a Brazilian low-cost airline based in Rio de Janeiro, Brazil. The airline's previous logo used from 2001-2015 ![]() official Swiss chronometer certification, 28,800 vph/4 Hz frequency, full balance bridge and free-sprung balance, overcoil hairspring,įunctions: hours, minutes, hacking seconds chronograph Movement: automatic Caliber 4130 with column wheel and vertical clutch chronograph, 72-hour power resreve, C.O.S.C. ![]() Quick Facts Rolex Oyster Perpetual Cosmograph Daytona 116500LN-0001Ĭase: 40 x 12.3 mm, stainless steel, 100-meter water resistance with screw-down crown Movement: automatic El Primero Caliber 3600 with lateral clutch column wheel chronograph, 60-hour power reserve, 36,000 vph/5 Hz frequency, silicon escapement, flat hairspring, 10-second foudroyantįunctions: hours, minutes, hacking seconds date, foudroyant chronograph The Chronomaster Sport is Swiss engineering’s equivalent of present-day Lynyrd Skynyrd: neither a cover act nor all original blood, but still a blast.įor more information, please visit Quick Facts Zenith Chronomaster Sport 03.3100.3600/21.M3100Ĭase: 41 x 13.8 mm, stainless steel, 100-meter water resistance with push-down crown With the benefit of wrist time, head-to-head comparison, and reflection, a more balanced view of the Zenith puts the contentious watch in perspective. And, no, the Rolex isn’t four times better than the Zenith.įrom its January launch, the Chronomaster Sport’s appearance has drawn immediate comparisons to the Rolex Daytona. The Zenith, by comparison, is available both new and used for approximately its retail price. Reality bites in the form of years-long dealer waiting lists for the Rolex and markups approaching $40,000 to buy the watch immediately preowned. As of October 2021, the steel Rolex Daytona retails for $13,150, and the Zenith Chronomaster Sport retails for $9,500 on a strap or $10,000 on a bracelet. Instead, we have a watch-nerd brand looking to find a fashion footing in a world where its celebrated movements often struggle for aesthetic identity. But Zenith isn’t Omega, and the Chronomaster Sport won’t cost Rolex a single Daytona sale. Rolex Cosmograph Daytona (left) and Zenith Chronomaster SportĪ larger watch brand or a more direct market rival to Rolex probably wouldn’t have risked a watch like the Chronomaster Sport. All due regard to the danger of small sample sizes, but most collectors who encountered the Chronomaster Sport came away impressed and convinced that the watch is more of a Daytona rival than a Daytona knockoff. Everyone wants to see it, try it, and judge it for themselves. Many watch collectors associate this subjectively beefy feel with luxury, and the Sport’s wrist feel is more tangible than the lighter Daytona.Ĭompliments from watch folks come fast and furious, and the Chronomaster Sport’s controversial origins make it a rare attention magnet from low-profile Zenith. There’s a reassuring solidity to the Chronomaster Sport, which benefits from its relatively chunky proportions and considerable weight. On the wrist, it’s easy to forget about the clasp, and the fidgety bracelet need be sized only once. Constructed of thin gauge-stamped steel and externally perforated for sizing adjustments, this clasp looks and feels like a relic of the last century or something suitable for a sub-$2K sports watch in 2021. Ironically, the Zenith clasp feels like a throwback to the Oyster clasps of the 1990s – the era of the Zenith-powered Daytona. Not only is this the design element most directly drawn from Rolex, but the quality feels out of step with the remainder of the Chronomaster. The deployant clasp is the low point of this Zenith. ![]() That means owners will require a specialized jig or Philippe Dufour-level dexterity with twin flatheads to size their bracelets without leaving scars.īracelets and deployant clasps of the Zenith Chronomaster Sport (left) and Rolex Cosmograph Daytona Although the three-link Chronomaster Sport bracelet is solid enough and all links are removable, the counter-torqued links include screw heads on both sides. Zenith’s design trades heavily on Oyster precedent, but the result falls short. The Zenith, by comparison, looks just as sharp in either configuration.Īs strong as the Chronomaster Sport is, the weak link lies among the links of the bracelet. While aftermarket firms are happy to assist, Rolex never intended for the 116500LN generation of the Cosmograph Daytona to include a strap, and swapping to a non-OEM solution often looks awkward. The Chronomaster Sport’s factory strap offering puts more daylight between it and the Daytona. Hands and indices are unique to the model and reflect a clear break with anything happening on Rue François-Dussaud. A 4:30 date indicator hews closely to El Primero history, and the overlapping tri-tone registers at center are straight off the Zenith A386 of 1969. The colors take clear cues from The Crown in Geneva, but the details are all Le Locle. Zenith offers both white and black dial options – which does nothing to discourage the Rolex comparisons. ![]() Unit Leader to collect and retain Annual Health and Medical Records. ![]() Unit Events: Unit Leader must submit the documents below to the Ranger at check-in. Ben Brown: 46 Luke Bach: 81 Required at Check-In.Sunday: Open - 7:00am, Close - 12:00pm (noon)įor access outside of these hours you MUST contact the camp Ranger:.Unit check-in and gate opening times for all camps are as follows: If no one is there, please use the radio to contact the camp ranger. Water will not be turned on and off based on the weather so please be aware that water may not be available in all campsites and bathrooms during these dates.Water will be available year-round at the Dining Hall and Bath Houses.Ĭheck in is at the little building located at the fork in the road as you enter camp. Important: Water in campsites and bathrooms may be turned off starting November 15 through March 1. 2-3 vehicles will be allowed to park NEAR your campsite all others vehicles MUST be moved to the Parking lot.Īll range fees MUST be paid for at the time of booking.Īny deposits paid will be refunded after check out, with verification from Camp Ranger that facilities were clean, undamaged and all equipment is inventoried.Generators are not to be run in Circle Ten Camps.Running extension cords to tents is prohibited.The batteries may be charged at available power outlets. ![]() CPAP Machines must be battery operated.It is the responsibility of the unit to have appropriate trained leaders for each activity and facility rental. To ensure the safest camping experience, all persons utilizing our camps must follow the guidelines set forth in the Guide to Safe Scouting and Age-Appropriate Guidelines.No late reservations or walk-ins will be accepted. 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